Zorbax 300StableBond
Zorbax 300Stablebond HPLC Columns from Agilent Technologies
Agilent ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC-MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers.
These columns are available in five different bonded phases (C18, C8, C3, Diphenyl and CN) for selectivity and recovery optimization of proteins and polypeptides. To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80 °C. Capillary and nano columns can be used for either 1-D or 2-D proteomics separations.
All pictures shown are for illustration purpose only. Actual product may vary.
- Buy
- Features
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- Four different bonded phases for reversed-phase selectivity optimization of peptides and proteins
- Exceptional stability and long lifetime at low pH with TFA containing mobile phases
- Excellent reproducibility
- Available in capillary and nano column dimensions for proteomics and other LC-MS applications
- Specifications
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300SB-C18 300SB-C8 300SB-C3 300SB-CN 300-Diphenyl Pore Size 300 Å 300 Å 300 Å 300 Å 300 Å Surface Area 45 m2/g 45 m2/g 45 m2/g 45 m2/g 45 m2/g Temp Limits* 90 ºC 80 ºC 80 ºC 80 ºC 80 ºC pH Range* 1 - 8 1 - 8 1 - 8 1 - 8 1 - 8 Endcapped No No No No Yes Carbon Load 2.8% 1.5% 1.1% 1.2% 1.9% USP Designation L1 L7 L56 L10 L11 * 300StableBond columns are designed for optimal use at low pH. At pH 6 - 8, highest column stability for all silica-based columns is obtained by operating at temperatures <40 °C and using low buffer concentrations in the range of 0.01 - 0.02 M
- Literature
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Reversed Phase Analysis of Proteins
Protein Identification and Impurity Profiling using Reversed-Phase HPLC/UHPLC
Agilent biocolumns can boost your accuracy and productivity. This document covers complete portfolio of reversed-phase columns for protein identity applications.
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Agilent Bio Column Selection Guide
Guidance on column selection for Biochromatography from Agilent
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